1. Statistical Reference Guide
  2. Reference interval

Transferring reference limitsTransfer a reference limit or reference interval from one method or laboratory to another.

  1. Select a cell in the dataset.
  2. On the Analyse-it ribbon tab, in the Statistical Analyses group, click Diagnostic, and then under the Reference Interval heading, click Transference.
    The analysis task pane opens.
  3. In the Y drop-down list, select the variable.
  4. Optional: To split the reference values by factors such as gender or age:
    1. On the Analyse-it ribbon tab, in the Reference Interval group, click Partition
    2. In the Factors list, select the factor variables.
    3. In the Partitions list, select a partition.

      The tasks change to the analysis options for that partition.

    4. Optional: By default, the analysis options are the same for each partition, set the specific analysis options for the partition as required.
    5. Repeat steps 4.c through 4.d for each partition.
  5. In the Reference level and Reference interval edit boxes, type the reference level as a percentage and the upper/lower reference limits.
  6. Optional: If transferring from another measurement procedure, under the Linear transformation heading, in the Constant and Proportional edit boxes, type the constant and proportional average bias (available from the method comparison study) to translate the old reference limits to the new measurement procedure.
  7. Select the Hypothesis test check box.
  8. Optional: In the Hypotheses drop-down list, select the null and alternative hypotheses.

    The default null hypothesis is that the proportion of observations in the reference interval is greater than or equal to the reference level, against the alternative hypothesis that it is less. A reference interval fails to transfer when too many observations fall outside it, so the one-sided test is the appropriate test.

  9. Optional: In the Significance level edit box, type the maximum probability of rejecting the null hypothesis when in fact it is true (default 10%).
    Note: CLSI EP28-A3 accepts the reference interval if no more than 2 of 20 samples fall outside the limits. The default one-sided hypothesis test at the 10% significance level reproduces this rule.
  10. If you selected partition factors, in the Partitions list, select another partition, and then repeat steps 5 through 9 for all partitions.
  11. Click Calculate.
Available in
Method Validation Edition Medical Edition Ultimate Edition

From the Statistical Reference Guide for Analyse-it 6.24.0: https://analyse-it.com/docs/user-guide/reference-interval/transfering-reference-limits

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