Test if the value of the reference material is equal to the assigned value; that is the bias is zero.
It is possible for the bias from a study to be different to 0, but for the difference to be due to the random error in your study. The smaller your study, the larger the bias has to be to be declared statistically significant. Also, just because bias is statistically significant does not mean that it is of concern, it may still meet your allowable bias performance requirements.
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