We are receiving a lot of questions about relevant analyses in the Analyse-it Method Validation edition to help in evaluating new diagnostic tests in the fight against COVID-19. Below are some quick links that will help, but contact us if you have questions - we are working as normal.

CLSI EP12-A2 for diagnostic accuracy see Sensitivity/Specificity, Predictive values, Estimating the sensitivity and specificity of a binary test and Comparing the accuracy of two binary diagnostic test.
CLSI EP17-A2 for detection capability of molecular methods using Probit analysis, see: Estimating the detection limit using a Probit fit and may be useful for determining the cutoff of a method with an internal continuous response see: Estimating the detection limit of a measurement system and Estimating the detection limit of a measurement system using a precision profile.

Also see our latest blog post: Sensitivity/Specificity and The Importance of Predictive Values for a COVID-19 test

EP12-A2 Example: Sensitivity specificity of 2 diagnostic tests

EP17-A2 Example 1:
LoB and LoD

EP17-A2 Example 2:
LoD from precision profile

EP17-A2 Example 4:
Probit detection limit

14-Oct-2013 Analyse-it Method Validation edition version 3.5 is now available

Today we released the Analyse-it Method Validation edition version 3.5. The software is feature complete, validated, and includes documentation. It supports Excel 2007, Excel 2010 (32- and 64-bit) and Excel 2013 (32- and 64-bit).

We took this opportunity to rename the product from the Analyse-it Method Evaluation edition to the Method Validation edition. The product is the same, but the new name better reflects the intended purpose of the product.

New features include:

Method Comparison

All method comparison functions: scatters plots, difference plots, and fits, are now in one place so it’s easy to make decisions or choose fits after looking at the data.
Ordinary linear, Weighted linear, Ordinary Deming, Weighted Deming and Passing & Bablok regressions.
Constant difference and relative difference plots with mean difference, allowable difference, and limits of agreement.
Bootstrap confidence intervals for parameters and bias in Passing & Bablok regression.
Exact p-values on CUSUM linearity test.
Predict mean difference at decision levels.
Equality and equivalence hypothesis tests at decision levels.
Kappa and Weighted Kappa qualitative method comparison.

Precision

Graybill-Wang / Ting-Burdick-Graybill-Jeyaratnam-Lu modified large sample confidence interval estimators for variance components – produces confidence closer to the stated level than Satterthwaite confidence intervals, which can be too liberal.
Support for unbalanced 1- and 2-factor nested random ANOVA models, for looking at precision between days, runs, laboratories, etc.

Linearity

Hsieh-Liu confidence interval estimator for degree of nonlinearity.
Equality and equivalence hypothesis tests for nonlinearity.

Reference interval

Harrell-Davis, Bootstrap, and Robust Bi-weight quantiles.
Box-Cox and other transformations.

Diagnostic performance / ROC

Compare up to 10 independent or correlated ROC curves.
Equality, equivalence and non-inferiority hypothesis tests.
Estimate false positive fraction (FPF) at fixed sensitivity, sensitivity at fixed FPF, and sensitivity/FPF at fixed cut-off.
Find optimal decision threshold based on costs.

Binary diagnostic tests

Compare sensitivity and specificity of 2 independent or correlated tests.
Diagnostic odds-ratio and Youden’s index.
Improved confidence interval estimators for likelihood ratio and predictive values.

For more information about the new version, and to download a free 30-day trial, see:

/products/method-validation

Pricing for the Analyse-it Method Validation edition starts at US$ 699 for a 1-user perpetual licence. If you already have a licence you may qualify for a free upgrade, if you have active maintenance, otherwise you can extend maintenance to get the upgrade (and all updates for 1- or 3-years) free of charge. To see if you qualify for a free upgrade, otherwise get a quote to extend maintenance, see:

/support/download

Comments are now closed.