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  • Statistical Reference Guide
  • Method comparison / Agreement

Plotting a difference plot and estimating the average bias

Plot a difference plot and estimate the average bias (average difference) between the methods.

  1. Select a cell in the dataset.
  2. On the Analyse-it ribbon tab, in the Statistical Analyses group, click Method Comparison, and then click:
    Option Description
    Mean difference Estimate the average difference using the mean of the differences.
    Median difference Estimate the average difference using the median of the differences. Useful when the distribution of the differences is not normally distributed
    The analysis task pane opens.
  3. If the data are in 2 variables:
    1. In the X drop-down list, select the comparative or reference measurement procedure variable.
    2. In the Y drop-down list, select the test measurement procedure variable.
    Note: If the variables consist of replicate measurements, select the variable name that spans all the replicate columns.
  4. If the data are in 2 variables with a separate variable identifying replicates of each item:
    1. In the X drop-down list, select the comparative or reference measurement procedure variable.
    2. In the Y drop-down list, select the test measurement procedure variable.
    3. In the Item drop-down list, select the variable identifying each item.
  5. If the data are in a single variable with a separate variable matching each item and a variable identifying the method:
    1. In the Model drop-down menu, select Matched Pairs.
    2. In the Y drop-down list, select the measurement variable.
    3. In the Item drop-down list, select the item variable that identifies each item.
    4. In the Method drop-down list, select the method variable.
  6. In the D drop-down list, select:
    Option Description
    Difference The differences are not related to magnitude.
    Relative Difference The differences are related to magnitude.
  7. In the Z drop-down list, select:
    Option Description
    X X is a reference method.
    (X+Y)/2 Neither X or Y are reference methods.
  8. Click Calculate.
Related concepts
Fit differences
Related tasks
Partitioning and reducing the measuring interval
Related reference
Dataset layout
Related information
CLSI. (2002). Method Comparison and Bias Estimation Using Patient Samples (EP9-A2). Clinical and Laboratory Standards Institute.
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  •  Estimating the bias between methods at a decision level
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  •  Difference plot (Bland-Altman plot)
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  •  Plotting a difference plot and estimating the average bias
  •  Limits of agreement (LoA)
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Version 6.15
Published 18-Apr-2023
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